Evaluation of antioxidant potential, DNA damage and hepatoprotective properties of Lagenaria siceraria plant against acetaminophen induced hepatotoxicity

Introduction: Lagenaria siceraria has various pharmacological effects like antioxidant, anti-hyperglycemic, anti-ulcer, anti-cancer, hepatoprotective, antimicrobial, anti-hyperlipidemic, anti-inflammatory and cardioprotective activities is used to treat diseases diabetes, jaundice, piles, ulcers, hypertension, colitis, insanity, skin diseases.Context purpose of the study: The aim present study was evaluate phytochemical content, antioxidant activity, DNA damage hepatoprotective (LS)plant’s parts explore their nutraceutical value.Methods: Phytochemical content measured by estimation total phenolics, carotenoids, ascorbic acid, protein carbohydrates while were investigated assaying parameters free radical scavenging lipid peroxidation, superoxide anion reducing power, protection assay. Hepatoprotective activity LS leaf cell viability, dismutase malondialdehyde nitric oxide formation.Results: Among tested plant samples, leaves showed high contents whereas fruits have carbohydrate contents. Evaluation in different that properties as compared other parts. L. also against Fenton’s reagent well UV induced hydroxyl radical’s damage. To test acetaminophen, hepatocellular toxicity extract concentrations 2.5, 5.0 7.5 μg/104 cells administered cultured hepatocytes before acetaminophen exposure. experimental results restores reduces formation alone treated cells.Conclusion: Our findings suggest plants potential damage-protective activities. Hence, part this may be food applications provide nutritional health benefits, liver care herbal formulations.Keywords: Antioxidant, Hepatocytes, Lipid Total phenolics